کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1929293 1050451 2012 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Role of ferritin in the cytodifferentiation of periodontal ligament cells
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Role of ferritin in the cytodifferentiation of periodontal ligament cells
چکیده انگلیسی

This study investigated the expression and functions of ferritin, which is involved in osteoblastogenesis, in the periodontal ligament (PDL). The PDL is one of the most important tissues for maintaining the homeostasis of teeth and tooth-supporting tissues. Real-time PCR analyses of the human PDL revealed abundant expression of ferritin light polypeptide (FTL) and ferritin heavy polypeptide (FTH), which encode the highly-conserved iron storage protein, ferritin. Immunohistochemical staining demonstrated predominant expression of FTL and FTH in mouse PDL tissues in vivo. In in vitro-maintained mouse PDL cells, FTL and FTH expressions were upregulated at both the mRNA and protein levels during the course of cytodifferentiation and mineralization. Interestingly, stimulation of PDL cells with exogenous apoferritin (iron-free ferritin) increased calcified nodule formation and alkaline phosphatase activity as well as the mRNA expressions of mineralization-related genes during the course of cytodifferentiation. On the other hand, RNA interference of FTH inhibited the mineralized nodule formation of PDL cells. This is the first report to demonstrate that ferritin is predominantly expressed in PDL tissues and positively regulates the cytodifferentiation and mineralization of PDL cells.


► Ferritin is abundantly expressed in the periodontal ligament (PDL).
► Ferritin positively regulates PDL cell cytodifferentiation and mineralization.
► Ferritin may be involved in PDL tissue homeostasis, remodeling and regeneration.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 426, Issue 4, 5 October 2012, Pages 643–648
نویسندگان
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