کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1929455 | 1050459 | 2012 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Characterization of human lung cancer-associated fibroblasts in three-dimensional in vitro co-culture model Characterization of human lung cancer-associated fibroblasts in three-dimensional in vitro co-culture model](/preview/png/1929455.png)
Lung cancer is the most common cause of cancer-related death worldwide. Stromal cancer-associated fibroblasts (CAFs) play crucial roles in carcinogenesis, proliferation, invasion, and metastasis of non-small cell lung carcinoma, and targeting of CAFs could be a novel strategy for cancer treatment. However, the characteristics of human CAFs still remain to be better defined. In this study, we established patient-matched CAFs and normal fibroblasts (NFs), from tumoral and non-tumoral portions of resected lung tissue from lung cancer patients. CAFs showed higher α-smooth muscle actin (α-SMA) expression than NFs, and CAFs clearly enhanced collagen gel contraction. Furthermore, we employed three-dimensional co-culture assay with A549 lung cancer cells, where CAFs were more potent in inducing collagen gel contraction. Hematoxylin and eosin staining of co-cultured collagen gel revealed that CAFs had the potential to increase invasion of A549 cells compared to NFs. These observations provide evidence that lung CAFs have the tumor-promoting capacity distinct from NFs.
► We established three patient-paired sets of CAFs and NFs.
► CAFs and NFs were analyzed using three-dimensional co-culture experiments.
► CAFs clearly enhanced collagen gel contraction.
► CAFs showed higher α-SMA expression than NFs.
► CAFs were implicated in invasion and differentiation of lung cancer cells.
Journal: Biochemical and Biophysical Research Communications - Volume 423, Issue 1, 22 June 2012, Pages 158–163