Characterization of p75+ ectomesenchymal stem cells from rat embryonic facial process tissue

Several populations of stem cells, including those from the dental pulp and periodontal ligament, have been isolated from different parts of the tooth and periodontium. The characteristics of such stem cells have been reported as well. However, as a common progenitor of these cells, ectomesenchymal stem cells (EMSCs), derived from the cranial neural crest have yet to be fully characterized. The aim of this study was to better understand the characteristics of EMSCs isolated from rat embryonic facial processes. Immunohistochemical staining showed that EMSCs had migrated to rat facial processes at E11.5, while the absence of epithelial invagination or tooth-like epithelium suggested that any epithelial–mesenchymal interactions were limited at this stage. The p75 neurotrophin receptor (p75NTR), a typical neural crest marker, was used to select p75NTR-positive EMSCs (p75+ EMSCs), which were found to show a homogeneous fibroblast-like morphology and little change in the growth curve, proliferation capacity, and cell phenotype during cell passage. They also displayed the capacity to differentiate into diverse cell types under chemically defined conditions in vitro. p75+ EMSCs proved to be homogeneous, stable in vitro and potentially capable of multiple lineages, suggesting their potential for application in dental or orofacial tissue engineering.

► Ectomesenchymal stem cells (EMSCs) were found to migrate to rat facial processes at E11.5.
► We successfully sorted p75NTR positive EMSCs (p75+ EMSCs).
► p75+ EMSCs up to nine passages showed relative stable proliferative activity.
► We examined the in vitro multilineage potential of p75+ EMSCs.
► p75+EMSCs provide an in vitro model for tooth morphogenesis.