کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1929511 1050462 2012 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Downregulation of the osmolyte transporters SMIT and BGT1 by AMP-activated protein kinase
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Downregulation of the osmolyte transporters SMIT and BGT1 by AMP-activated protein kinase
چکیده انگلیسی

The myoinositol transporter SMIT (SLC5A3) and the betaine/γ-aminobutyric acid (GABA) transporter BGT1 (SLC6A12) accomplish cellular accumulation of organic osmolytes and thus contribute to cell volume regulation. Challenges of cell volume constancy include energy depletion, which compromises the function of the Na+/K+ ATPase leading to cellular Na+ accumulation and subsequent cell swelling. Energy depletion is sensed by AMP-activated protein kinase (AMPK). The present study explored whether AMPK influences the activity of SMIT and BGT1. To this end, cRNA encoding SMIT or BGT1 was injected into Xenopus oocytes with and without additional injection of wild type AMPK (AMPKα1 + AMPKβ1 + AMPKγ1), of constitutively active γR70QAMPK (AMPKα1 + AMPKβ1 + R70QAMPKγ1) or of catalytically inactive αK45RAMPK (K45RAMPKα1 + AMPKβ1 + AMPKγ1). Substrate-induced current in dual electrode voltage-clamp experiments was taken as measure of osmolyte transport. As a result, in SMIT-expressing, but not in water-injected Xenopus oocytes, myoinositol, added to the extracellular bath, generated a current (ISMIT), which was half maximal (KM) at ≈7.2 μM myoinositol concentration. Furthermore, in BGT1-expressing, but not in water-injected Xenopus oocytes, GABA added to the bath generated a current (IGABA), which was half maximal (KM) at ≈0.5 mM GABA concentration. Coexpression of AMPK and of γR70QAMPK but not of αK45RAMPK significantly decreased ISMIT and IGABA. AMPK decreased the respective maximal currents without significantly modifying the respective KM. In conclusion, the AMP-activated kinase AMPK is a powerful regulator of the organic osmolyte transporters SMIT and BGT1 and thus interacts with cell volume regulation.


► AMP-activated kinase AMPK inhibits the Na+, myoinositol transporter SMIT (SLC5A3).
► AMPK inhibits the betaine/γ-aminobutyric acid (GABA) transporter BGT1 (SLC6A12).
► The effect of AMPK is mimicked by active γR70QAMPK but not by inactive K45RAMPK.
► AMPK decreases the maximal transport rate without affecting the affinity of the carriers.
► AMPK thus participates in the regulation of osmolyte transport and of cell volume.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 422, Issue 3, 8 June 2012, Pages 358–362
نویسندگان
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