کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1929558 1536782 2012 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Stress granules contribute to α-globin homeostasis in differentiating erythroid cells
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Stress granules contribute to α-globin homeostasis in differentiating erythroid cells
چکیده انگلیسی

Hemoglobin is the major biosynthetic product of developing erythroid cells. Assembly of hemoglobin requires the balanced production of globin proteins and the oxygen-carrying heme moiety. The heme-regulated inhibitor kinase (HRI) participates in this process by phosphorylating eIF2α and inhibiting the translation of globin proteins when levels of free heme are limiting. HRI is also activated in erythroid cells subjected to oxidative stress. Phospho-eIF2α-mediated translational repression induces the assembly of stress granules (SG), cytoplasmic foci that harbor untranslated mRNAs and promote the survival of cells subjected to adverse environmental conditions. We have found that differentiating erythroid, but not myelomonocytic or megakaryocytic, murine and human progenitor cells assemble SGs, in vitro and in vivo. Targeted knockdown of HRI or G3BP, a protein required for SG assembly, inhibits spontaneous and arsenite-induced assembly of SGs in erythroid progenitor cells. This is accompanied by reduced α-globin production and increased apoptosis suggesting that G3BP+ SGs facilitate the survival of developing erythroid cells.


► A subset of differentiating erythroid cells assemble spontaneous stress granules.
► Stress granule assembly is a consequence of HRI-induced phosphorylation of eIF2α.
► Knockdown of G3BP inhibits stress granule assembly and erythroid cell survival.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 420, Issue 4, 20 April 2012, Pages 768–774
نویسندگان
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