کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1929739 | 1050472 | 2012 | 7 صفحه PDF | دانلود رایگان |
The mushroom known as Reishi (Ganoderma lucidum) has been used as an herbal medicine for tumor treatment and immune system activation. Because its effects on the differentiation of effector T helper cells have not yet been fully understood, we investigated the effects of Reishi and those of its principal ingredient, β-glucan, on the activation of dendritic cells and the differentiation of Th17 cells. Reishi extracts as well as purified β-glucan (Curdran) activated DCs and caused them to produce large amounts of IL-23. β-glucan also enhanced and sustained the transcription of IL-23p19. The MEK-ERK signaling pathway positively regulates IL-23p19 transcription in β-glucan-stimulated DCs. In a mixed leukocyte reaction, Reishi-stimulated DCs preferentially induced Th17 cells. Furthermore, orally-administrated Reishi increased the percentages of Th17 cells and the transcription levels of antimicrobial peptides. Our results show that Reishi and β-glucan activate DCs to produce large amounts of IL-23, which induces Th17 differentiation both in vitro and in vivo.
► Reishi and β-glucan (Curdran) induce large amounts of IL-23 but not IL-12 from dendritic cells.
► β-Glucan enhanced and sustained the transcription of IL-23p19 through the MEK-ERK pathway.
► Reishi-stimulated DCs preferentially induced Th17 cells.
► Oral administration of Reishi increased the percentages of Th17 cells in vivo.
Journal: Biochemical and Biophysical Research Communications - Volume 422, Issue 1, 25 May 2012, Pages 174–180