کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1929859 | 1050476 | 2012 | 7 صفحه PDF | دانلود رایگان |

Among approximately 480 RING domain-containing E3 Ub ligases in Arabidopsis, three, At3g46620, At5g59550, and At2g39720, have a domain-of-unknown-function (DUF) 1117 motif in their C-terminal regions. At3g46620 and At5g59550 were identified as homologous ABA- and drought-induced RING-DUF1117 genes and were designated AtRDUF1 and AtRDUF2, respectively. Single and double knock-out mutations of AtRDUFs resulted in hyposensitive phenotypes toward ABA in terms of germination rate and stomatal closure and markedly reduced tolerance to drought stress relative to wild-type plants. These results are discussed in the context that AtRDUF1 and AtRDUF2 play combinatorial, but still distinguishable, roles in ABA-mediated dehydration stress responses.
► Arabidopsis contains three RING-DUF1117 E3 ubiquitin ligases.
► AtRDUF1 and AtRDUF2 were identified as ABA- and drought-induced RING-DUF1117 genes.
► Loss-of-function mutations of AtRDUFs resulted in hyposensitive phenotypes to ABA.
► Knock-out mutations of AtRDUFs markedly reduced tolerance to drought stress.
► AtRDUFs may play redundant, but still distinguishable, roles in drought responses.
Journal: Biochemical and Biophysical Research Communications - Volume 420, Issue 1, 30 March 2012, Pages 141–147