کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1929867 | 1050476 | 2012 | 5 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Crystal structure of the Rasputin NTF2-like domain from Drosophila melanogaster Crystal structure of the Rasputin NTF2-like domain from Drosophila melanogaster](/preview/png/1929867.png)
The crystal structure of the NTF2-like domain of the Drosophila homolog of Ras GTPase SH3 Binding Protein (G3BP), Rasputin, was determined at 2.7 Å resolution. The overall structure is highly similar to nuclear transport factor 2: It is a homodimer comprised of a β-sheet and three α-helices forming a cone-like shape. However, known binding sites for RanGDP and FxFG containing peptides show electrostatic and steric differences compared to nuclear transport factor 2. A HEPES molecule bound in the structure suggests a new, and possibly physiologically relevant, ligand binding site.
► The crystal structure of the NTF2-like domain of Rasputin protein is presented.
► Differences to known ligand binding sites of nuclear transport factor 2 are discussed.
► A new ligand binding site for the Rasputin and G3BP proteins is proposed.
Journal: Biochemical and Biophysical Research Communications - Volume 420, Issue 1, 30 March 2012, Pages 188–192