کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1929892 | 1050477 | 2012 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: IL-3 promotes osteoblast differentiation and bone formation in human mesenchymal stem cells IL-3 promotes osteoblast differentiation and bone formation in human mesenchymal stem cells](/preview/png/1929892.png)
IL-3 is an important cytokine that regulates hematopoiesis. We have previously demonstrated that IL-3 is a potent inhibitor of osteoclastogenesis and bone resorption. In the present study, we have investigated the role of IL-3 on human osteoblast differentiation and bone formation. We found that IL-3 in a dose-dependent manner increases osteoblast differentiation and matrix mineralization in human mesenchymal stem cells (MSCs). IL-3 significantly enhances the expression of osteoblast specific genes such as alkaline phosphatase, collagen type-I, osteocalcin and osteopontin; and Runx-2 and osterix transcription factors. Moreover, IL-3 induces the expression of bone morphogenetic protein-2 (BMP-2), and activates smad1/5/8. IL-3 enhances osteoblast differentiation and BMP-2 secretion through JAK/STAT pathway. Interestingly, IL-3 promotes in vivo bone regeneration ability of MSCs. Thus, we reveal for the first time that IL-3 enhances human osteoblast differentiation and bone formation in both in vitro and in vivo conditions, and suggest its therapeutic potential for bone formation in important bone diseases.
► IL-3 enhances human osteoblast differentiation in mesenchymal stem cells.
► IL-3 increases osteogenesis through bone morphogenetic protein-2.
► IL-3 promotes the in vivo bone regeneration ability of human MSCs.
► IL-3 may be useful as an anabolic agent for bone formation in bone diseases.
Journal: Biochemical and Biophysical Research Communications - Volume 418, Issue 4, 24 February 2012, Pages 669–675