کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1929914 | 1050477 | 2012 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Expression of Notch receptors and ligands on immature and mature T cells Expression of Notch receptors and ligands on immature and mature T cells](/preview/png/1929914.png)
Notch plays multiple roles in T cell development in the thymus and T cell differentiation in the periphery. In order to systematically examine the role of Notch in T cell biology, we determined the cell surface expression of all Notch receptors and ligands on various populations of T cells by using a panel of specific monoclonal antibodies we recently established. Notch1 and Notch3 were upregulated at double-negative (DN) 2-DN4 stages of immature thymocytes, then downregulated on mature single-positive thymocytes and peripheral T cells, but were rapidly upregulated again upon activation. Notch2 was consistently expressed on T cells while Notch4 was not. Jagged1 and Jagged2 were expressed at double-positive stage of immature T cells. Jagged2 was also inducible on mature T cells upon activation. In contrast, no Delta-like (Dll) 1 or Dll4 expression was observed on T cells. These comprehensive profiling of the expression of Notch receptors and ligands would be informative to fully understand the role of individual Notch receptors and ligands in T cell development and differentiation.
► Notch2 and Notch3 as well as Notch1 were expressed on immature thymocytes.
► Jagged1 and Jagged2 were expressed on CD4+CD8+ thymocytes.
► Activation of mature T cells upregulated Notch1, Notch2, Notch3, and Jagged2.
► No differences in Notch receptor/ligand expression among effector T cell subsets.
Journal: Biochemical and Biophysical Research Communications - Volume 418, Issue 4, 24 February 2012, Pages 799–805