کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1929975 1050486 2011 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Mutation of calcineurin subunit B M118 influences the activities of NF-AT and p53, but not calcineurin expression level
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Mutation of calcineurin subunit B M118 influences the activities of NF-AT and p53, but not calcineurin expression level
چکیده انگلیسی

Calcineurin (CN) is a Ca2+/calmodulin-dependent phosphatase, which consists of a catalytic A-subunit (CnA) and a regulatory B-subunit (CnB). Endogenous CnA and CnB have a strong corelationship in cancer cell lines. Through the introduction of CnB and its mutants in cells, we show that CnB does not increase the expression of CnA but protects it from degradation. CnB M118 is necessary for tight binding to CnA. Point mutations of CnB M118 also do not increase the expression of CnA but protect it from degradation. Furthermore, CnB M118K fails to enhance the activities of NF-AT and p53 induced by CnA in HeLa-s cells. Mutations in CnB M118 may prove to be a valuable marker in the diagnostics of some important illnesses such as Alzheimer’s disease.


► Endogenous CnA and CnB have a statistically significant relationship.
► Point mutation of CnB M118 influences its interaction with subunit A.
► CnB M118K does not change the expression level of CnA, but decreases its activity.
► Point mutation CnB M118 may act a marker in diagnosing and curing some diseases.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 413, Issue 3, 30 September 2011, Pages 481–486
نویسندگان
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