کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1930090 1050489 2012 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
mHERC6 is the essential ISG15 E3 ligase in the murine system
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
mHERC6 is the essential ISG15 E3 ligase in the murine system
چکیده انگلیسی

Posttranslational protein modification by ubiquitin and ubiquitin-like modifiers (UBLs) is mediated by a hierarchical cascade of conjugating enzymes and affects multiple biological processes within the cell. Interferon-stimulated gene 15 (ISG15) is an UBL, which is strongly induced by type I Interferon and ISG15 modification was shown to play an essential role in antiviral defense. While hHERC5 is the major E3 ligase for ISG15 modification in humans, ISGylation in the murine systems at the level of E3 ligases was weakly characterized as rodent genomes lack a direct homologue of hHERC5.Here, we show that mHERC6 is strongly induced by different pathogen-associated molecular patterns (PAMPs) in a type I Interferon receptor (IFNAR1) dependent manner. We demonstrate that mHERC6 is essential for endogenous murine ISGylation and thus represents the dominant ISG15 E3 ligase in mice. In contrast to its human homologue, mHERC6 is also capable to mediate conjugation of human ISG15.


► The HECT domain containing protein mHERC6 is essential for ISGylation in mice.
► mHERC6 induction by different PAMPs depends on type I Interferon signaling.
► mHERC6 is crossreactive and efficiently conjugates human and murine ISG15.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 417, Issue 1, 6 January 2012, Pages 135–140
نویسندگان
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