کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1930133 | 1050489 | 2012 | 6 صفحه PDF | دانلود رایگان |
Female flowers of hop (Humulus lupulus L.) develop a large number of glandular trichomes called lupulin glands that contain a variety of prenylated compounds such as α- and β-acid (humulone and lupulone, respectively), as well as xanthohumol, a chalcone derivative. These prenylated compounds are biosynthesized by prenyltransferases catalyzing the transfer of dimethylallyl moiety to aromatic substances. In our previous work, we found HlPT-1 a candidate gene for such a prenyltransferase in a cDNA library constructed from lupulin-enriched flower tissues. In this study, we have characterized the enzymatic properties of HlPT-1 using a recombinant protein expressed in baculovirus-infected insect cells. HlPT-1 catalyzed the first transfer of dimethylallyl moiety to phloroglucinol derivatives, phlorisovalerophenone, phlorisobutyrophenone and phlormethylbutanophenone, leading to the formation of humulone and lupulone derivatives. HlPT-1 also recognized naringenin chalcone as a flavonoid substrate to yield xanthohumol, and this broad substrate specificity is a unique character of HlPT-1 that is not seen in other reported flavonoid prenyltransferases, all of which show strict specificity for their aromatic substrates. Moreover, unlike other aromatic substrate prenyltransferases, HlPT-1 revealed an exclusive requirement for Mg2+ as a divalent cation for its enzymatic activity and also showed exceptionally narrow optimum pH at around pH 7.0.
► HlPT-1 is a membrane-bound prenyltransferase in hops.
► HlPT-1 catalyzes the first prenylation step for humulone and lupulone biosynthesis.
► HlPT-1 shows broad substrate specificity ranging from phloroglucinol to chalcone.
► This enzyme shows many unique biochemical features unlike other prenyltransferase.
Journal: Biochemical and Biophysical Research Communications - Volume 417, Issue 1, 6 January 2012, Pages 393–398