Stau1 regulates Dvl2 expression during myoblast differentiation

Post-transcriptional regulation of gene expression by RNA-binding proteins has pivotal roles in many biological processes. We have shown that Stau1, a conserved RNA-binding protein, negatively regulates myogenesis in C2C12 myoblasts. However, its target mRNAs in regulation of myogenesis remain unknown. Here we describe that Stau1 positively regulates expression of Dvl2 gene encoding a central mediator of Wnt pathway in undifferentiated C2C12 myoblasts. Stau1 binds to 3′ untranslated region (UTR) of Dvl2 mRNA and Stau1 knockdown shortened a half-life of the mRNA containing Dvl2 3′ UTR. After induction of myogenic differentiation, association of Stau1 with 3′ UTR of Dvl2 mRNA was decreased. Correlated with the decrease in the association, the Dvl2 mRNA level was reduced during myogenesis. A forced expression of Dvl2 markedly inhibited progression of myogenic differentiation. Our results suggest that Dvl2 has an inhibitory role in myogenesis and Stau1 coordinates myogenesis through the regulation of Dvl2 mRNA.

► Stau1 binds to the 3′ untranslated region (UTR) of Dvl2 mRNA in C2C12 myoblasts.
► Stau1 regulates the stability of mRNA containing 3′ UTR of Dvl2 mRNA.
► Stau1 dissociates from Dvl2 mRNA following induction of myogenesis.
► Dvl2 has an inhibitory role in myoblast differentiation.
► Stau1 coordinates myogenesis by regulating Dvl2 expression.