کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1930189 1050491 2011 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Myostatin induces autophagy in skeletal muscle in vitro
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Myostatin induces autophagy in skeletal muscle in vitro
چکیده انگلیسی

Myostatin is an important regulator of muscle mass that contributes to the loss of muscle mass in a number of chronic diseases. Myostatin is known to activate the expression of components of the ubiquitin-proteosomal pathway but its effect on the autophagic pathway is not known. We therefore analysed the effect of myostatin and TGF-β on autophagy in C2C12 cells by determining the effect of these proteins on LC3 processing, autophagosome formation and autophagy gene expression. Both myostatin and TGF-β increased LC3II expression and turnover as well as autophagosome formation (marked by the formation of puncta in LC3-GFP transfected cells). Myostatin also significantly increased the expression of ATG-4B and ULK-2 mRNA while TGF-β caused a trend towards an increase in these genes. We conclude that myostatin and TGF-β increase autophagy in skeletal muscle cells.


► Myostatin increases LC3 processing and LC3II turnover in muscle cells.
► Myostatin increases the formation of LC3 positive puncta in muscle cells.
► Myostatin increases the expression of genes associated with autophagy.
► These data indicate that myostatin induces autophagy in muscle cells.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 415, Issue 4, 2 December 2011, Pages 632–636
نویسندگان
, , ,