An epifluorescence microscopy method for generalized polarization imaging

Generalized polarization (GP) microscopy represents an excellent tool to study lipid–lipid and lipid–protein interactions in situ and in vitro. Here, we present an efficient and cost effective method to perform GP microscopy using a standard light-emitting diode (LED) epifluorescence microscope equipped with a digital color camera.

Figure optionsDownload as PowerPoint slideHighlights
► We created an epifluorescence method for generalized polarization (GP) microscopy.
► Our method is based on fluorescence ratio imaging using a RGB additive color camera.
► Intensity adjustable LED excitation minimizes photobleaching.
► The sensitivity and reproducibility of epifluorescence GP microscopy are demonstrated.
► Qualitative and quantitative GP measurements of model membranes are demonstrated.