Study of reciprocal effects of cardiac myosin and tropomyosin isoforms on actin–myosin interaction with in vitro motility assay

Interaction of myosin with actin in striated muscle is controlled by Ca2+ via thin filament associated proteins: troponin and tropomyosin. In cardiac muscle there is a whole pattern of myosin and tropomyosin isoforms. The aim of the current work is to study regulatory effect of tropomyosin on sliding velocity of actin filaments in the in vitro motility assay over cardiac isomyosins. It was found that tropomyosins of different content of α- and β-chains being added to actin filament effects the sliding velocity of filaments in different ways. On the other hand the velocity of filaments with the same tropomyosins depends on both heavy and light chains isoforms of cardiac myosin.

► A whole pattern of myosin and tropomyosin isoforms in cardiac muscle.
► In the in vitro motility assay tropomyosin isoforms effect the sliding velocity of filaments in different ways.
► The velocity of filaments with the same tropomyosins depends on both heavy and light chains of myosin isoforms.