کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1930362 | 1050505 | 2011 | 6 صفحه PDF | دانلود رایگان |

Lipin-1 plays a crucial role in the regulation of lipid metabolism and cell differentiation in adipocytes. Expression of adipose lipin-1 is reduced in obesity, and metabolic syndrome. However, the significance of this reduction remains unclear. This study investigated if and how reduced lipin-1 expression affected metabolism. We assessed mRNA expression levels of various genes related to adipocyte metabolism in lipin-1-depleted 3T3-L1 adipocytes by introducing its specific small interfering RNA. In lipin-1-depleted adipocytes, mRNA and protein expression levels of monocyte chemoattractant protein-1 (MCP-1) were significantly increased, although the other genes tested were not altered. The conditioned media from the cells promoted monocyte chemotaxis. The increase in MCP-1 expression was prevented by treatment with quinazoline or salicylate, inhibitors of nuclear factor-κB activation. Because MCP-1 is related to adipose inflammation and systemic insulin resistance, these results suggest that a reduction in adipose lipin-1 in obesity may exacerbate adipose inflammation and metabolism.
► Lipin-1 affects lipid metabolism, adipocyte differentiation, and transcription.
► Adipose lipin-1 expression is reduced in obesity.
► Lipin-1 depletion using siRNA in 3T3-L1 adipocytes increased MCP-1 expression.
► Lipin-1 is involved in adipose inflammation.
Journal: Biochemical and Biophysical Research Communications - Volume 415, Issue 1, 11 November 2011, Pages 200–205