کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1930622 | 1050520 | 2011 | 6 صفحه PDF | دانلود رایگان |
Leukocyte-associated immunoglobulin-like receptor-1 (LAIR-1) is an inhibitory collagen receptor which belongs to the immunoglobulin (Ig) superfamily. Although the inhibitory function of LAIR-1 has been extensively described in multiple leukocytes, its role in megakaryocyte (MK) has not been explored so far. Here, we show that LAIR-1 is expressed on human bone marrow CD34+CD41a+ and CD41a+CD42b+ cells. LAIR-1 is also detectable in a fraction of human cord blood CD34+ cell-derived MK that has morphological characteristics of immature MK. In megakaryoblastic cell line Dami, the membrane protein expression of LAIR-1 is up-regulated significantly when cells are treated with phorbol ester phorbol 12-myristate 13-acetate (PMA). Furthermore, cross-linking of LAIR-1 in Dami cells with its natural ligand or anti-LAIR-1 antibody leads to the inhibition of cell proliferation and PMA-promoted differentiation when examined by the MK lineage-specific markers (CD41a and CD42b) and polyploidization. In addition, we also observed that cross-linking of LAIR-1 results in decreased MK generation from primary human CD34+ cells cultured in a cytokines cocktail that contains TPO. These results suggest that LAIR-1 is a likely candidate for an early marker of MK differentiation, and provide initial evidence indicating that LAIR-1 serves as a negative regulator of megakaryocytopoiesis.
Research highlights
► LAIR-1 is expressed on human megakaryocytes from an early stage.
► Up-regulation of LAIR-1 negatively regulates megakaryocytic differentiation of cell line.
► LAIR-1 negatively regulates the differentiation of primary megakaryocytic progenitors.
Journal: Biochemical and Biophysical Research Communications - Volume 405, Issue 1, 4 February 2011, Pages 128–133