کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1930651 1050521 2011 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
PICOT is a molecule which binds to anamorsin
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
PICOT is a molecule which binds to anamorsin
چکیده انگلیسی

Anamorsin (AM) (also called CIAPIN-1) is a cell-death-defying factor. AM deficient mice die during late gestation; AM deficient embryos are anemic and very small compared to wild type (WT) embryos. It is thought that AM plays crucial roles in hematopoiesis and embryogenesis. To clarify the mechanisms of AM functions, we performed the yeast-two-hybrid assay to identify AM-interacting molecules; we found that PICOT (PKCθ interacting cousin of thioredoxin) preferentially bound to AM. We also showed that the N-terminal regions of both AM and PICOT were essential for their bindings and the inhibition of interaction of both molecules might lead to the cell growth retardation. Both PICOT and the yeast homolog of AM are known to be iron–sulfur proteins. The phenotype of PICOT deficient mice is very similar to that of anamorsin deficient mice; both mice are embryonic lethal. These data suggest that AM and PICOT might play cooperatively essential roles in embryogenesis as iron–sulfur cluster proteins.


► Anamorsin (AM) (also called CIAPIN-1) is a cell-death-defying factor.
► Biological mechanisms of AM functions have not been elucidated yet.
► We identified PICOT preferentially bound to AM.
► The N-terminal regions of both AM and PICOT were essential for their bindings.
► Both PICOT and the yeast homolog of AM are known to be iron–sulfur proteins.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 408, Issue 2, 6 May 2011, Pages 329–333
نویسندگان
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