کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1930671 1050523 2011 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Efficient mutation identification in zebrafish by microarray capturing and next generation sequencing
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Efficient mutation identification in zebrafish by microarray capturing and next generation sequencing
چکیده انگلیسی

Fish models like medaka, stickleback or zebrafish provide a valuable resource to study vertebrate genes. However, finding genetic variants e.g. mutations in the genome is still arduous. Here we used a combination of microarray capturing and next generation sequencing to identify the affected gene in the mozartkugelp11cv (mzlp11cv) mutant zebrafish. We discovered a 31-bp deletion in macf1 demonstrating the potential of this technique to efficiently isolate mutations in a vertebrate genome.

Research highlights
► The maternal-effect mutation mozartkugelp11cv shows an embryonic polarity defect.
► The mutation localizes to a 770 kb interval on zebrafish chromosome 19.
► DNA capturing and next-generation sequencing identifies a genomic deletion.
► The mutation causes a premature STOP-codon deleting 2483 amino acids of Macf1.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 405, Issue 3, 18 February 2011, Pages 373–376
نویسندگان
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