کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1930671 | 1050523 | 2011 | 4 صفحه PDF | دانلود رایگان |

Fish models like medaka, stickleback or zebrafish provide a valuable resource to study vertebrate genes. However, finding genetic variants e.g. mutations in the genome is still arduous. Here we used a combination of microarray capturing and next generation sequencing to identify the affected gene in the mozartkugelp11cv (mzlp11cv) mutant zebrafish. We discovered a 31-bp deletion in macf1 demonstrating the potential of this technique to efficiently isolate mutations in a vertebrate genome.
Research highlights
► The maternal-effect mutation mozartkugelp11cv shows an embryonic polarity defect.
► The mutation localizes to a 770 kb interval on zebrafish chromosome 19.
► DNA capturing and next-generation sequencing identifies a genomic deletion.
► The mutation causes a premature STOP-codon deleting 2483 amino acids of Macf1.
Journal: Biochemical and Biophysical Research Communications - Volume 405, Issue 3, 18 February 2011, Pages 373–376