کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1930688 | 1050523 | 2011 | 5 صفحه PDF | دانلود رایگان |
The calcineurin (CN) B subunit (CNB) is the regulatory subunit of CN, which is the only serine/threonine-specific protein phosphatase regulated by Ca2+/CaM. It has been shown to have potential as an anticancer agent, and has a positive effect on the phagocytic index and coefficient. We report here that CNB binds to proteasome subunit alpha type 7 (PSMA7) and inhibits the transactivation activity of hypoxia-inducible factor-1α (HIF-1α) via the proteasome pathway. In addition, we show that CNB represses the expression of vascular endothelial growth factor (VEGF), which is regulated by HIF-1α. These results indicate that CNB modulates cellular proteasome activity via a specific interaction with PSMA7. This may provide a molecular basis for its anticancer and antiviral activities.
Research highlights
► CNB binds to PSMA7 and regulates the gating effect of PSMA7 on proteasomal activity.
► By stimulating proteasomal activity CNB inhibits HIF-1α transactivation activity.
► CNB represses transcription of VEGF.
► These observations suggest a molecular basis for the anticancer and antiviral actions of CNB.
Journal: Biochemical and Biophysical Research Communications - Volume 405, Issue 3, 18 February 2011, Pages 468–472