کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1930750 1050526 2011 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Induction of yeast apoptosis by an antimicrobial peptide, Papiliocin
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Induction of yeast apoptosis by an antimicrobial peptide, Papiliocin
چکیده انگلیسی

Papiliocin is a 37-residue peptide isolated from the swallowtail butterfly Papilio xuthus. In this study, we found that Papiliocin induced the accumulation of reactive oxygen species (ROS) and hydroxyl radicals known to be important regulators of apoptosis in Candida albicans. To examine the relationship between the accumulation of ROS and the induction of apoptosis, we investigated the apoptotic effects of Papiliocin using apoptotic markers. Cells treated with Papiliocin showed a series of cellular changes normally seen in cells undergoing apoptosis: plasma membrane translocation of phosphatidylserine from the inner to the outer membrane leaflet, measured by Annexin V staining, dissipation of the mitochondrial membrane potential, observed by DiOC6(3) staining; and the presence of active metacaspases, measured using the CaspACE FITC-VAD-FMK, as early apoptotic events. In addition, DNA condensation and fragmentation, which is important marker of late stage apoptosis, was seen by DAPI and TUNEL assay. Therefore, these results suggest that Papiliocin leads to apoptosis in C. albicans via ROS accumulation.


► Papiliocin induces an increase in the total intracellular levels of ROS, specifically hydroxyl radicals.
► Papiliocin induces exposure of phosphatidylserine at the cytoplasmic membrane and mitochondrial membrane depolarization.
► Papiliocin induces the activation of metacaspases.
► Papiliocin induces DNA damage, a features of a late apoptosis.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 408, Issue 1, 29 April 2011, Pages 89–93
نویسندگان
, , , , , , ,