کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1930777 | 1050527 | 2011 | 5 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Zyxin phosphorylation at serine 142 modulates the zyxin head–tail interaction to alter cell–cell adhesion Zyxin phosphorylation at serine 142 modulates the zyxin head–tail interaction to alter cell–cell adhesion](/preview/png/1930777.png)
Zyxin is an actin regulatory protein that is concentrated at sites of actin–membrane association, particularly cell junctions. Zyxin participates in actin dynamics by binding VASP, an interaction that occurs via proline-rich N-terminal ActA repeats. An intramolecular association of the N-terminal LIM domains at or near the ActA repeats can prevent VASP and other binding partners from binding full-length zyxin. Such a head–tail interaction likely accounts for how zyxin function in actin dynamics, cell adhesion, and cell migration can be regulated by the cell. Since zyxin binding to several partners, via the LIM domains, requires phosphorylation, it seems likely that zyxin phosphorylation might alter the head–tail interaction and, thus, zyxin activity. Here we show that zyxin point mutants at a known phosphorylation site, serine 142, alter the ability of a zyxin fragment to directly bind a separate zyxin LIM domains fragment protein. Further, expression of the zyxin phosphomimetic mutant results in increased localization to cell–cell contacts of MDCK cells and generates a cellular phenotype, namely inability to disassemble cell–cell contacts, precisely like that produced by expression of zyxin mutants that lack the entire regulatory LIM domain region. These data suggest that zyxin phosphorylation at serine 142 results in release of the head–tail interaction, changing zyxin activity at cell–cell contacts.
Research highlights
► A phosphomimetic mutation at zyxin serine 142 prevents the zyxin head–tail interaction.
► Zyxin mutations at serine 142 do not alter zyxin localization to cell–cell junction.
► The phosphomimetic mutation at zyxin serine 142 prevents cell–cell detachment.
► A phosphorylation-based model for controlling the zyxin activity at cell–cell junctions is proposed.
Journal: Biochemical and Biophysical Research Communications - Volume 404, Issue 3, 21 January 2011, Pages 780–784