کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1930854 1050532 2011 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The roles of transforming growth factor-β and Smad3 signaling in adipocyte differentiation and obesity
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
The roles of transforming growth factor-β and Smad3 signaling in adipocyte differentiation and obesity
چکیده انگلیسی

We aimed at elucidating the roles of transforming growth factor (TGF)-β and Smad3 signaling in adipocyte differentiation (adipogenesis) and in the pathogenesis of obesity. TGF-β/Smad3 signaling in white adipose tissue (WAT) was determined in genetically obese (ob/ob) mice. The effect of TGF-β on adipogenesis was evaluated in mouse embryonic fibroblasts (MEF) isolated both from WT controls and Smad3 KO mice by Oil red-O staining and gene expression analysis. Phenotypic analyses of high-fat diet (HFD)-induced obesity in Smad3 KO mice compared to WT controls were performed. TGF-β/Smad3 signaling was elevated in WAT from ob/ob mice compared to the controls. TGF-β significantly inhibited adipogenesis in MEF, but the inhibitory effects of TGF-β on adipogenesis were partially abolished in MEF from Smad3 KO mice. TGF-β inhibited adipogenesis independent from the Wnt and β-catenin pathway. Smad3 KO mice were protected against HFD-induced insulin resistance. The size of adipocytes from Smad3 KO mice on the HFD was significantly smaller compared to the controls. In conclusion, the TGF-β/Smad3 signaling pathway plays key roles not only in adipogenesis but also in development of insulin resistance.


► The expression of the Smad3 was increased in white adipose tissue from ob/ob mice.
► TGF-β inhibited adipogenesis partially through the Smad3-dependent pathway.
► Smad3 KO mice were protected against high-fat diet (HFD)-induced insulin resistance. Under the HFD, the adipocytes from Smad3 KO mice were smaller than the WT controls.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 407, Issue 1, 1 April 2011, Pages 68–73
نویسندگان
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