کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1930948 | 1050535 | 2010 | 6 صفحه PDF | دانلود رایگان |
Trichosanthin (TCS) is a type I ribosome-inactivating protein that inhibits the replication of both human immunodeficiency virus type 1 (HIV-1) and herpes simplex virus type 1 (HSV-1). The mechanism of inhibition is not clear. This investigation explored the effects of TCS on the stages of HSV-1 infection in Hep-2 cells, from attachment to release. We demonstrated that TCS reduced HSV-1 antigen and DNA content and interfered with viral replication as early as 3–15 h after infection. TCS had no effect on HSV-1 attachment, penetration or immediate-early gene expression. However, the expression of early and late genes and virion release were diminished. In summary, this study demonstrates that TCS primarily affects HSV-1 replication in Hep-2 cells during the early to late infection period.
Research highlights
► TCS decreases HSV-1 antigen.
► TCS interferes with viral replication between 3 and 15 h after infection.
► TCS affects HSV-1 early to late gene expression.
► TCS interferes HSV-1 package and release.
Journal: Biochemical and Biophysical Research Communications - Volume 402, Issue 4, 26 November 2010, Pages 670–675