14-3-3η inhibits chondrogenic differentiation of ATDC5 cell

It was previously shown that 14-3-3η is overexpressed in the synovial fluid of patients with joint inflammation, which is often associated with growth failure. In this study, we investigated the role of 14-3-3η in chondrogenesis using ATDC5 cells. Upon treatment with TNF-α, cells overexpressed 14-3-3η with inhibition of chondrogenesis. Chondrogenesis was also inhibited by overexpression of 14-3-3η without TNF-α treatment, whereas silencing of 14-3-3η promoted chondrogenic differentiation. Further, G1 phase arrest was inhibited by overexpression of 14-3-3η. In summary, we suggest that 14-3-3η plays a regulatory role in chondrogenic differentiation.

Research highlights
► Upon treatment with TNF-α, cells overexpressed 14-3-3η with inhibition of chondrogenesis.
► Chondrogenesis was also inhibited by overexpression of 14-3-3η without TNF-α treatment.
► Silencing of 14-3-3η promoted chondrogenic differentiation.
► G1 phase arrest was inhibited by overexpression of 14-3-3η.
► In summary, we suggest that 14-3-3η plays a regulatory role in chondrogenic differentiation.