Unconventional actin conformations localize on intermediate filaments in mitosis

► Unconventional actin conformations colocalize with vimentin on a cage-like structure in metaphase HEK 293T cells. ► These conformations are detected with the anti-actin antibodies 1C7 ('lower dimer') and 2G2 ('nuclear actin'), but not C4 (monomeric actin). ► Mitotic unconventional actin cables are independent of filamentous actin or microtubules. ► Unconventional actin colocalizes with vimentin on a nocodazole-induced perinuclear dense mass of cables.