کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1931164 1050543 2011 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The melatonin action on stromal stem cells within pericryptal area in colon cancer model under constant light
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
The melatonin action on stromal stem cells within pericryptal area in colon cancer model under constant light
چکیده انگلیسی

Constant light (LL) is associated with high incidence of colon cancer. MLT supplementation was related to the significant control of preneoplastic patterns. We sought to analyze preneoplastic patterns in colon tissue from animals exposed to LL environment (14 days; 300 lx), MLT-supplementation (10 mg/kg/day) and DMH-treatment (1,2 dimethylhydrazine; 125 mg/kg). Rodents were sacrificed and MLT serum levels were measured by radioimmunoassay. Our results indicated that LL induced ACF development (p < 0.001) with a great potential to increase the number of CD133(+) and CD68(+) cells (p < 0.05 and p < 0.001). LL also increased the proliferative process (PCNA-Li; p < 0.001) as well as decreased caspase-3 protein (p < 0.001), related to higher COX-2 protein expression (p < 0.001) within pericryptal colonic stroma (PCCS). However, MLT-supplementation controlled the development of dysplastic ACF (p < 0.001) diminishing preneoplastic patterns into PCCS as CD133 and CD68 (p < 0.05 and p < 0.001). These events were relative to decreased PCNA-Li index and higher expression of caspase-3 protein. Thus, MLT showed a great potential to control the preneoplastic patterns induced by LL.

Research highlights
► We investigated melatonin against the malignant effects of constant light.
► Melatonin supplementation increased its serum levels and its receptor expression.
► Melatonin decreased cancer stem cells and dysplastic injuries in colon tissue.
► Melatonin controlled proliferative process and apoptosis induction.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 405, Issue 4, 25 February 2011, Pages 593–598
نویسندگان
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