کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1931319 1050548 2010 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Molecular cloning and characterization of Cup a 4, a new allergen from Cupressus arizonica
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Molecular cloning and characterization of Cup a 4, a new allergen from Cupressus arizonica
چکیده انگلیسی

Sensitization to Cupressaceae pollen has become one of the most important causes of pollinosis in Western countries during winter and early spring. However, the characterization of the extracts, the allergens involved and the cross-reactivity with other pollen sources still remain poorly studied; in the case of Cupressus arizonica only two allergens have been described so far. A new allergen from C. arizonica pollen, Cup a 4, was cloned and expressed in Escherichia coli as an N-terminally His-tag recombinant protein that was characterized biochemically, immunologically and by circular dichroism spectroscopy. The new allergen has high sequence identity with Prickly Juniper allergen Jun o 4 and contains four EF-hand domains. The recombinant protein has structural similarities with other calcium binding allergens such as Ole e 3, Ole e 8 and Phl p 7. Cup a 4 is expressed in mature pollen grains and shares antigenic properties with the recombinant form. Sera from 9.6% C. arizonica allergic patients contain specific IgE antibodies against recombinant Cup a 4.

Research highlights
► Cup a 4 is a new allergen from the Arizona cypress.
► Cup a 4 is expressed in mature pollen grains.
► Cup a 4 contains four EF-hand domains and has calcium binding properties.
► rCup a 4 binds IgE from patients allergic to Cupressus arizonica pollen.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 401, Issue 3, 22 October 2010, Pages 451–457
نویسندگان
, , , , , , , , ,