کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1931365 1536787 2010 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Efficient production and purification of functional bacteriorhodopsin with a wheat-germ cell-free system and a combination of Fos-choline and CHAPS detergents
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Efficient production and purification of functional bacteriorhodopsin with a wheat-germ cell-free system and a combination of Fos-choline and CHAPS detergents
چکیده انگلیسی

Cell-free translation is one potential approach to the production of functional transmembrane proteins. We have now examined various detergents as supplements to a wheat-germ cell-free system in order to optimize the production and subsequent purification of a functional model transmembrane protein, bacteriorhodopsin. We found that Fos-choline and CHAPS detergents counteracted each other’s inhibitory effects on cell-free translation activity and thereby allowed the efficient production and subsequent purification of functional bacteriorhodopsin in high yield.

Research highlights
► Efficient production of functional bacteriorhodopsin with a wheat-germ cell-free system.
► Finding of unique combination of detergents, Fos-choline and CHAPS.
► The detergent mix is compatible with a procedure for the protein purification.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 400, Issue 4, 1 October 2010, Pages 638–642
نویسندگان
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