کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1931365 | 1536787 | 2010 | 5 صفحه PDF | دانلود رایگان |

Cell-free translation is one potential approach to the production of functional transmembrane proteins. We have now examined various detergents as supplements to a wheat-germ cell-free system in order to optimize the production and subsequent purification of a functional model transmembrane protein, bacteriorhodopsin. We found that Fos-choline and CHAPS detergents counteracted each other’s inhibitory effects on cell-free translation activity and thereby allowed the efficient production and subsequent purification of functional bacteriorhodopsin in high yield.
Research highlights
► Efficient production of functional bacteriorhodopsin with a wheat-germ cell-free system.
► Finding of unique combination of detergents, Fos-choline and CHAPS.
► The detergent mix is compatible with a procedure for the protein purification.
Journal: Biochemical and Biophysical Research Communications - Volume 400, Issue 4, 1 October 2010, Pages 638–642