کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1931400 1050551 2010 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Generation and characterization of T1R2-LacZ knock-in mouse
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Generation and characterization of T1R2-LacZ knock-in mouse
چکیده انگلیسی

Taste cells are chemosensory epithelial cells that sense distinct taste quality such as umami, sweet, bitter, sour and salty. Taste cells utilize G protein-coupled receptors to detect umami, sweet and bitter taste whereas ion channels are responsible for detecting salty and sour taste. Among these taste receptors, taste receptor type 2, T1R2 (or Tas1r2), has been identified as a sole sweet taste receptor in mammals that mediates sweet signals upon dimerization with T1R3. However, because of limited availability of reliable antibodies and low expression level of G protein-coupled receptors, it is uneasy to identify the cell-types that express these receptors in non-taste tissues. In this study, we have generated a T1R2-LacZ reporter knock-in mouse to investigate tissue distribution of T1R2 at a single-cell level. We found that the LacZ gene expression in these mice was faithful to the expression of T1R2 in the taste tissue and in the gastrointestinal tract where T1R3 expression has been reported. Surprisingly, T1R2 expression was also found in the testis. Mice homozygous for T1R2 deletion lacked T1R2 protein analyzed by the antibody raised against T1R2 peptide sequences. In summary, the T1R2 knock-in mouse is a powerful tool to analyze the putative targets for sweeteners as well as to study the physiological roles of T1R2 in detecting sugars.

Research highlights
► The sweet taste receptor knock-in mouse, T1R2-LacZ mouse, was generated.
► The reporter expression was faithful to the T1R2 expression in the taste bud.
► We also confirmed the reporter gene expression in the intestine and the testis.
► This is the first report for knock-in mouse of the taste receptor.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 402, Issue 3, 19 November 2010, Pages 495–499
نویسندگان
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