Adipose tissue-derived stem cells secrete CXCL5 cytokine with chemoattractant and angiogenic properties

Adipose tissue-derived stem cells (ADSC) secreted CXCL5 cytokine abundantly and higher passaged ADSC up to passage 6 (P6) secreted more CXCL5 than lower passaged ADSC. Higher passaged ADSC also appeared to express higher levels of CXCL5 receptor, i.e., CXCR2. Both CXCL5 and CXCR2 were localized in the tunica intima and tunica adventitia of blood vessels in adipose tissue. Colocalization with CD34 further indicates their association with the putative ADSC in tunica adventitia. Migration assay indicates chemoattractant effects of CXCL5 on ADSC and HUVEC endothelial cells. CXCL5 also enhanced matrigel-based endothelial tube-like formation of HUVEC.

Research highlights
► Adipose tissue-derived stem cells (ADSC) secrete CXCL5 cytokine abundantly.
► ADSC also express higher levels of CXCL5 receptor, i.e., CXCR2.
► Both CXCL5 and CXCR2 were localized in the tunica intima and tunica adventitia of blood vessels in adipose tissue.
► CXCL5 has chemoattractant effects on ADSC and HUVEC endothelial cells.
► CXCL5 also enhanced matrigel-based endothelial tube-like formation of HUVEC.