کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1931453 1050553 2010 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Human CD133-derived bone marrow stromal cells establish ectopic hematopoietic microenvironments in immunodeficient mice
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Human CD133-derived bone marrow stromal cells establish ectopic hematopoietic microenvironments in immunodeficient mice
چکیده انگلیسی

Cultured adherent bone marrow stromal cells (BMSCs) are capable of forming ectopic hematopoietic microenvironments (HMEs) in immunodeficient mice. However, the cell surface phenotype of the native bone marrow stem/progenitor cell that gives rise to BMSCs that support hematopoiesis remains poorly defined. We recently reported the derivation of human BMSC-like cells (CD133BMSCs) by magnetic cell sorting against Prominin-1 (CD133), an epitope expressed by embryonic, fetal, and adult stem cells. Here we demonstrate that CD133BMSCs are capable of forming ectopic HMEs. Cultured adherent CD133BMSCs derived from sorted CD133-positive cells lacked CD133 expression, but were uniformly positive for CD146, an epitope recently described to identify self-renewing osteoprogenitor cells that could transfer the HME. CD133BMSCs were genetically-tagged by lentivirus, expanded, and seeded into HA/TCP/fibrin constructs that were implanted subcutaneously. After 60 days, CD133BMSCs produced human osteocytes, osteoblasts, adipocytes, and reticular cells that supported murine hematopoiesis. CD133BMSCs that were not transduced with lentivirus also formed HMEs. Control constructs seeded with human dermal fibroblasts formed connective tissue, but failed to form HMEs. Our data indicate that CD133 expression identifies a native human bone marrow stem/progenitor cell that gives rise to BMSCs capable of forming the HME.

Research highlights
► CD133-derived bone marrow stem cells can form ectopic hematopoietic niches in mice.
► CD133 identifies bone marrow stem cells that lie upstream of CD146-positive cells.
► CD133 is likely to identify the native human stem/progenitor cell in bone marrow.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 400, Issue 2, 17 September 2010, Pages 212–218
نویسندگان
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