کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1931545 1050557 2010 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Role of a PAS sensor domain in the Mycobacterium tuberculosis transcription regulator Rv1364c
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Role of a PAS sensor domain in the Mycobacterium tuberculosis transcription regulator Rv1364c
چکیده انگلیسی
The Mycobacterium tuberculosis transcriptional regulator Rv1364c regulates the activity of the stress response σ factor σF. This multi-domain protein has several components: a signaling PAS domain and an effector segment comprising of a phosphatase, a kinase and an anti-anti-σ factor domain. Based on Small Angle X-ray Scattering (SAXS) data, Rv1364c was recently shown to be a homo-dimer and adopt an elongated conformation in solution. The PAS domain could not be modeled into the structural envelope due to poor sequence similarity with known PAS proteins. The crystal structure of the PAS domain described here provides a structural basis for the dimerization of Rv1364c. It thus appears likely that the PAS domain regulates the anti-σ activity of Rv1364c by oligomerization. A structural comparison with other characterized PAS domains reveal several sequence and conformational features that could facilitate ligand binding - a feature which suggests that the function of Rv1364c could potentially be governed by specific cellular signals or metabolic cues.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 398, Issue 3, 30 July 2010, Pages 342-349
نویسندگان
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