ESR technique for noninvasive way to quantify cyclodextrins effect on cell membranes

A new way to study the action of cyclodextrin was developed to quantify the damage caused on cell membrane and lipid bilayer. The Electron Spin Resonance (ESR) spectroscopy was used to study the action of Randomly methylated-beta-cyclodextrin (Rameb) on living cells (HCT-116). The relative anisotropy observed in ESR spectrum of nitroxide spin probe (5-DSA and cholestane) is directly related to the rotational mobility of the probe, which can be further correlated with the microviscosity. The use of ESR probes clearly shows a close correlation between cholesterol contained in cells and cellular membrane microviscosity. This study also demonstrates the Rameb ability to extract cholesterol and phospholipids in time- and dose-dependent ways. In addition, ESR spectra enabled to establish that cholesterol is extracted from lipid rafts to form stable aggregates. The present work supports that ESR is an easy, reproducible and noninvasive technique to study the effect of cyclodextrins on cell membranes.

Research highlights
► ESR: a new tool for cyclodextrins study on living cells.
► Cholesterol and phospholipid extraction by Rameb in a dose- and time-dependent way.
► Extracted phospholipids and cholesterol form stable aggregates.
► ESR spectra show that lipid rafts are damaged by Rameb.
► Quantification of the cholesterol extraction on cell membranes in a noninvasive way.