کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1932032 | 1050572 | 2010 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Design and evaluation of a multi-epitope peptide against Japanese encephalitis virus infection in BALB/c mice Design and evaluation of a multi-epitope peptide against Japanese encephalitis virus infection in BALB/c mice](/preview/png/1932032.png)
Epitope-based vaccination is a promising means to achieve protective immunity and to avoid immunopathology in Japanese encephalitis virus (JEV) infection. Several B-cell and T-cell epitopes have been mapped to the E protein of JEV, and they are responsible for the elicitation of the neutralizing antibodies and CTLs that impart protective immunity to the host. In the present study, we optimized a proposed multi-epitope peptide (MEP) using an epitope-based vaccine strategy, which combined six B-cell epitopes (amino acid residues 75–92, 149–163, 258–285, 356–362, 373–399 and 397–403) and two T-cell epitopes (amino acid residues 60–68 and 436–445) from the E protein of JEV. This recombinant protein was expressed in Escherichia coli, named rMEP, and its protective efficacy against JEV infection was assessed in BALB/c mice. The results showed that rMEP was highly immunogenic and could elicit high titer neutralizing antibodies and cell-mediated immune responses. It provided complete protection against lethal challenge with JEV in mice. Our findings indicate that the multi-epitope vaccine rMEP may be an attractive candidate vaccine for the prevention of JEV infection.
Journal: Biochemical and Biophysical Research Communications - Volume 396, Issue 4, 11 June 2010, Pages 787–792