کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1932110 | 1050573 | 2010 | 5 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Construction, screening and identification of a phage display antibody library against the Eimeria acervulina merozoite Construction, screening and identification of a phage display antibody library against the Eimeria acervulina merozoite](/preview/png/1932110.png)
A single-chain antibody library against Eimeria acervulina merozoites was constructed by phage display approach. Antibody-displaying phage was selected in four panning rounds against cryopreserved E. acervulina merozoites. Five clones were randomly selected from the fourth panning round, and their nucleotide sequences were aligned and compared to mouse germ-line sequences. Soluble antibody was produced in a non-suppressor Escherichia coli strain, purified by protein A affinity chromatography, and characterized by Western-blotting. Immunofluorescence assay showed localization of the produced recombinant antibody fragment on the surface E. acervulina merozoites. These resultant antibody fragments showed high specificity and binding capacity for soluble antigens and intact fixed merozoites which seems promising as diagnostic, therapeutic and/or vaccine tools against coccidiosis.
Journal: Biochemical and Biophysical Research Communications - Volume 393, Issue 4, 19 March 2010, Pages 703–707