کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1932431 1050580 2010 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Inward-rectifier chloride currents in Reissner’s membrane epithelial cells
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Inward-rectifier chloride currents in Reissner’s membrane epithelial cells
چکیده انگلیسی

Sensory transduction in the cochlea depends on regulated ion secretion and absorption. Results of whole-organ experiments suggested that Reissner’s membrane may play a role in the control of luminal Cl−. We tested for the presence of Cl− transport pathways in isolated mouse Reissner’s membrane using whole-cell patch clamp recording and gene transcript analyses using RT-PCR. The current–voltage (I–V) relationship in the presence of symmetrical NMDG-Cl was strongly inward-rectifying at negative voltages, with a small outward current at positive voltages. The inward-rectifying component of the I–V curve had several properties similar to those of the ClC-2 Cl− channel. It was stimulated by extracellular acidity and inhibited by extracellular Cd2+, Zn2+ and intracellular ClC-2 antibody. Channel transcripts expressed include ClC-2, Slc26a7 and ClC-Ka, but not Cftr, ClC-1, ClCa1, ClCa2, ClCa3, ClCa4, Slc26a9, ClC-Kb, Best1, Best2, Best3 or the beta-subunit of ClC-K, barttin. ClC-2 is the only molecularly-identified channel present that is a strong inward rectifier. This study is the first report of conductive Cl− transport in epithelial cells of Reissner’s membrane and is consistent with an important role in endolymph anion homeostasis.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 394, Issue 2, 2 April 2010, Pages 434–438
نویسندگان
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