کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1932648 | 1050586 | 2010 | 5 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Cleavage-induced termination in U2 snRNA gene expression Cleavage-induced termination in U2 snRNA gene expression](/preview/png/1932648.png)
The maturation of many small nuclear RNAs is dependent on RNase III-like endonuclease mediated cleavage, which generates a loading site for the exosome complex that trims the precursor at its 3′ end. Using a temperature sensitive Pac1 nuclease, here we show that the endonuclease cleavage is equally important in terminating the transcription of the U2 snRNA in Schizosaccharomyces pombe. Using a temperature sensitive Dhp1p 5′ → 3′ exonuclease, we demonstrate that it also is an essential component of the termination pathway. Taken together the results support a “reversed torpedoes” model for the termination and maturation of the U2 snRNA; the Pac1 endonuclease cleavage provides entry sites for the 3′ and 5′ exonuclease activities, leading to RNA maturation in one direction and transcript termination in the other.
Journal: Biochemical and Biophysical Research Communications - Volume 393, Issue 3, 12 March 2010, Pages 461–465