کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1932718 | 1050588 | 2009 | 4 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Epigenetic modification of rhizobial genome is essential for efficient nodulation Epigenetic modification of rhizobial genome is essential for efficient nodulation](/preview/png/1932718.png)
CcrM is one of the solitary bacterial DNA methyltransferases which does not have corresponding restriction enzymes. We established a stable ccrM-overexpressing mutant of Mesorhizobium loti, MlccrM-OX, and performed molecular and phenotypic characterization of this strain. In the M. loti MlccrM-OX infected plants, nodulation was apparently delayed at 7 days after inoculation (dai), however, the nodules that eventually formed on the MlccrM-OX roots showed nitrogen fixing ability by at least 21 dai. These results suggest that the initial morphogenic events were affected by ccrM-overexpression and that the correct pattern of DNA methylation of the bacterial genome is not essential for plant–microbe symbiosis, but are required for efficient nodulation.
Journal: Biochemical and Biophysical Research Communications - Volume 389, Issue 2, 13 November 2009, Pages 301–304