کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1932724 1050588 2009 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Membrane cholesterol depletion from live cells enhances the function of human serotonin1A receptors
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Membrane cholesterol depletion from live cells enhances the function of human serotonin1A receptors
چکیده انگلیسی

Work from our laboratory has previously demonstrated the requirement of membrane cholesterol in the function of the serotonin1A receptor, a member of the G-protein coupled receptor (GPCR) superfamily. In order to monitor the effect of cellular organization on the function of human serotonin1A receptors, we explored receptor function following cholesterol depletion in live cells and membranes isolated from cholesterol-depleted cells. We report here the novel observation that while ligand binding of serotonin1A receptors displays an increase in membranes isolated from cholesterol-depleted cells, such trend is absent when binding is performed on cholesterol-depleted intact cells. Importantly, we show here, for the first time, that G-protein coupling of the serotonin1A receptor is enhanced in membranes isolated from cholesterol-depleted cells. These results assume pharmacological relevance in view of the recently described structural evidence of specific cholesterol binding sites in GPCRs, and may help in designing better therapeutic strategies for diseases related to GPCRs.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 389, Issue 2, 13 November 2009, Pages 333–337
نویسندگان
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