کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1933094 1050602 2009 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Crystal structure of the E. coli tRNAArg aminoacyl stem isoacceptor RR-1660 at 2.0 Å resolution
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Crystal structure of the E. coli tRNAArg aminoacyl stem isoacceptor RR-1660 at 2.0 Å resolution
چکیده انگلیسی

Due to the redundancy of the genetic code there exist six mRNA codons for arginine and several tRNAArg isoacceptors which translate these triplets to protein within the context of the mRNA. The tRNA identity elements assure the correct aminoacylation of the tRNA with the cognate amino acid by the aminoacyl-tRNA-synthetases. In tRNAArg, the identity elements consist of the anticodon, parts of the D-loop and the discriminator base. The minor groove of the acceptor stem interacts with the arginyl-tRNA-synthetase. We crystallized different Escherichia coli tRNAArg acceptor stem helices and solved the structure of the tRNAArg isoacceptor RR-1660 microhelix by X-ray structure analysis. The acceptor stem helix crystallizes in the space group P1 with the cell constants a = 26.28, b = 28.92, c = 29.00 Å, α = 105.74, β = 99.01, γ = 97.44° and two molecules per asymmetric unit. The RNA hydration pattern consists of 88 bound water molecules. Additionally, one glycerol molecule is bound within the interface of the two RNA molecules.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 385, Issue 1, 17 July 2009, Pages 84–87
نویسندگان
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