کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1933181 1050605 2009 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The large-conductance Ca2+-activated K+ channel interacts with the apolipoprotein ApoA1
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
The large-conductance Ca2+-activated K+ channel interacts with the apolipoprotein ApoA1
چکیده انگلیسی

Owing to the multifaceted functions of the large conductance Ca2+-activated K+ channel (BK), identification of protein–protein interactions is essential in determining BK regulation. A yeast two-hybrid screening of a cochlear cDNA library revealed a BK–ApoA1 interaction. Patch clamp recordings of excised membrane patches from transfected HEK293 cells showed that ApoA1 inhibits the BK α-subunit by significantly increasing activation and deactivation times, and shifting half-activation voltage to more positive potentials. Reciprocal coimmunoprecipitations verified the BK–ApoA1 interaction using excised sensory epithelium and ganglia. Additionally, immunocolocalization studies revealed BK and ApoA1 expression in both receptor cells and auditory neurons. These data suggest new avenues of investigation, given the importance of apolipoproteins in neurological diseases.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 387, Issue 4, 2 October 2009, Pages 671–675
نویسندگان
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