کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1933352 1050610 2009 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Treacle recruits RNA polymerase I complex to the nucleolus that is independent of UBF
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Treacle recruits RNA polymerase I complex to the nucleolus that is independent of UBF
چکیده انگلیسی

Mutations in treacle lead to Treacher Collins syndrome (TCS), an autosomal dominant disorder of craniofacial development. Treacle associates with upstream binding factor (UBF) to regulate rRNA gene (rDNA) transcription, but the precise mechanisms mediated by treacle remain elusive. Here we show that the central repeated domain of treacle binds with RNA polymerase I (Pol I), while that the treacle C-terminus is involved in rDNA promoter recognition and UBF recruitment. Knockdown of treacle resulted in dispersion of Pol I and UBF away from nucleolus, whereas interactions of treacle with Pol I and rDNA promoter were not disrupted by UBF depletion. These findings indicate that treacle, but not UBF, is essential for nucleolar recruitment of Pol I transcription complex. Furthermore, C-terminally truncated treacle, mimicking TCS-associated mutations, failed to target to the nucleolus, possibly causing loss-of-function in the mutant treacle. Our observations support that TCS results from haploinsufficiency of treacle.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 386, Issue 2, 21 August 2009, Pages 396–401
نویسندگان
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