کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1933399 | 1050612 | 2009 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Fetal hepatic expression of 5-lipoxygenase activating protein is confined to colonizing hematopoietic cells
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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چکیده انگلیسی
Leukotriene C4 is a potent inflammatory mediator formed from arachidonic acid and glutathione. 5-Lipoxygenase (5-LO), 5-lipoxygenase activating protein (FLAP) and leukotriene C4 synthase (LTC4S) participate in its biosynthesis. We report evidence from in situ hybridization experiments that FLAP mRNA is abundantly expressed in fetal mouse liver from e11.5 until delivery. In contrast very little or no FLAP mRNA was detected in adult liver. The fetal expression in liver was not uniform but occurred in patches. Cells from e15.5 livers were fractionated by fluorescence activated cell sorting into hepatocytes and other CD45â cells and CD45+ hematopoietic cells. The latter were further separated into immature (Linâ) and mature (Lin+) cells and analyzed for FLAP mRNA content by quantitative RT-PCR. FLAP mRNA expression was confined to CD45+ cells and the mature cells had approximately 4-fold higher FLAP mRNA levels compared to the immature cells.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 383, Issue 3, 5 June 2009, Pages 336-339
Journal: Biochemical and Biophysical Research Communications - Volume 383, Issue 3, 5 June 2009, Pages 336-339
نویسندگان
Tobias Strid, Cecilia Karlsson, Mats Söderström, Jie Zhang, Hong Qian, Mikael Sigvardsson, Sven Hammarström,