کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1933563 | 1050617 | 2009 | 5 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: DOC2b is a SNARE regulator of glucose-stimulated delayed insulin secretion DOC2b is a SNARE regulator of glucose-stimulated delayed insulin secretion](/preview/png/1933563.png)
Insulin secretion is precisely regulated by blood glucose with unique biphasic pattern. The regulatory mechanism of the second-phase insulin release is unclear. In this study, we report that DOC2b (double C2 domain protein isoform b), a SNARE related protein, was associated with insulin vesicles and translocated to plasma membrane within several minutes upon high-glucose stimulation followed by an interaction with syntaxin4, but not syntaxin1. This binding specificity and the time course of DOC2b translocation were suitable for the regulation of second-phase insulin release. Increased DOC2b expression enhanced glucose-stimulated insulin secretion. In contrast, silencing DOC2b inhibited delayed release of insulin, without affecting rapid (∼7 min) phase secretion. Interestingly, DOC2b had no effects on KCl-triggered insulin release. These data suggest that DOC2b may be a regulator for delayed (second-phase) insulin secretion in MIN6 cells.
Journal: Biochemical and Biophysical Research Communications - Volume 384, Issue 4, 10 July 2009, Pages 461–465