کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1933981 1050631 2009 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Investigation of requirements for efficient gene delivery using the HIV-1 based lentiviral transduction system
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Investigation of requirements for efficient gene delivery using the HIV-1 based lentiviral transduction system
چکیده انگلیسی

The specific recognition and selection of the HIV-1 packaging signal psi (Ψ) sequence which is mediated by Gag protein is believed to be pivotal for selective viral genomic RNA packaging and has been a basis for the development of HIV-based transgene delivery systems. However, the requirement of the psi sequence has been questioned recently by a report postulating that the psi element is not absolutely required for transgene transduction. Here, we used a four-plasmid transgene delivery system and analyzed the results by HIV p24 antigen assay, MT4 infection assay, HT1080 colony assay, and reverse transcription-PCR (RT-PCR). The results clearly demonstrate that the psi sequence must be present for efficient transgene encapsidation and transduction.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 383, Issue 2, 29 May 2009, Pages 192–197
نویسندگان
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