کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1934123 | 1050633 | 2008 | 5 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Cathepsin E regulates the presentation of tetanus toxin C-fragment in PMA activated primary human B cells Cathepsin E regulates the presentation of tetanus toxin C-fragment in PMA activated primary human B cells](/preview/png/1934123.png)
Processing of antigens by proteases in the endocytic compartments of antigen presenting cells (APC) is essential to make them suitable for presentation as antigenic peptides to T lymphocytes. Several proteases of the cysteine, aspartyl and serine classes are involved in this process. It has been speculated, that the aspartyl protease cathepsin E (CatE) is involved in antigen processing in B cell line, monocyte-derived dendritic cells (DC) and murine DC. Here we show the expression of CatE in primary human B cells and DC, which was only elevated in B cells after induction with phorbol 12-myristate 13-acetate (PMA), resulted in enhanced presentation of tetanus toxin C-fragment (TTC) to the respective T cells. Inhibition of aspartyl proteases using pepstatin-A-penetratin (PepA-P), a highly efficient, cell-permeable aspartyl protease inhibitor, reduced significantly T cell activation in PMA activated B cells but not in PMA activated myeloid DC (mDC). Thus we suggest that CatE is important in the processing of TTC in primary human B cells.
Journal: Biochemical and Biophysical Research Communications - Volume 377, Issue 4, 26 December 2008, Pages 1299–1303