کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1934176 | 1050634 | 2009 | 5 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Collagen type V modulates fibroblast behavior dependent on substrate stiffness Collagen type V modulates fibroblast behavior dependent on substrate stiffness](/preview/png/1934176.png)
Collagen type V is highly expressed during tissue development and wound repair, but its exact function remains unclear. Cell binding to collagen V affects various basic cell functions and increased collagen V levels alter the structural organization and the stiffness of the ECM. We studied the combined effects of collagen V and substrate stiffness on the morphology, focal adhesion formation, and actin organization of fibroblasts. We found that a hybrid collagen I/V coating impairs fibroblast spreading on soft substrates (<10 kPa), but not on stiffer substrates (68 kPa or glass). In sharp contrast, a pure collagen I coating does not impair cell spreading on soft substrates. The impairment of cell spreading by collagen V is accompanied by diffuse actin staining patterns and small focal adhesions. These observations suggest that collagen V plays an essential role in modifying cell behavior during development and remodeling, when very soft tissues are present.
Journal: Biochemical and Biophysical Research Communications - Volume 380, Issue 2, 6 March 2009, Pages 425–429